Grain growth 4 to 10 days

Endosperm cellularisation


This transverse section at 6 days after flowering shows just the large central embryo sac with the endosperm starting to form. Cell walls start to grow towards the central vacuole from just inside the nucellus.
Median transverse section at 5 days after flowering. The firmly structured pericarp surrounds the fluid-filled, but rapidly expanding, embryo sac. The first divisions of the endosperm nuclei result in a single layer of syncytial endosperm close to the nucellus.
The above section seen in Darkfield illumination. The pericarp is full of starch; the young endosperm has none.
The whole grain in longitudinal section at 5 days after flowering. endosperm nuclei divide rapidly within the central vacuole and produce the cellular endosperm near the embryo.
Median transverse section at 7 days after flowering. The first ring of syncytial endosperm has joined cells walls. Cell walls grow inwards from the periphery of the endosperm forming tube-like structures that compartmentalize nuclei and cytoplasm and later cross walls form. The centre of the endosperm is still unstructured.
Some endosperm nuclei have migrated to the nucellus and new cell walls are enclosing them. Other nuclei are still in free division. At high magnification the first wall ingrowths are seen. Cellularisation starts near the nucellar projection of the ventral groove and differentiation spreads laterally to the dorsal side of the grain.
Longitudinal section of the whole grain at 6 days after flowering. The free dividing coenocytic nuclei start to populate the rest of the embryo sac.